Thermo Fisher Scientific HA Epitope Tag Polyclonal Antibody, HRP
HA 태그 단백질 검출용 HRP 결합 토끼 다클론 항체. Western blot, IHC, ELISA, IP에 적합. 고순도 친화 크로마토그래피 정제. 4°C 단기 보관, -20°C 장기 보관 권장. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:15,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114–122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low abundance proteins when specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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