Thermo Fisher Scientific DDX1 Monoclonal Antibody (3E5E2)
DDX1 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합. Human 및 Mouse 반응성. Protein G 정제, PBS buffer에 0.05% sodium azide 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E5E2 |
| Immunogen | Purified recombinant fragment of human DDX1 (AA: 642–740) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at −20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787336 |
Product Specific Information
MA5-31712 has been tested in indirect ELISA.
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase capable of unwinding both RNA-RNA and RNA-DNA duplexes. It possesses 5′ single-stranded RNA overhang nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides.
DDX1, together with RELA, acts as a coactivator to enhance NF-κB-mediated transcriptional activation. It also positively regulates cyclin CCND2 expression by binding to its promoter region.
DDX1 is part of a multi-helicase–TICAM1 complex that serves as a cytoplasmic sensor of viral double-stranded RNA and contributes to antiviral response activation via TICAM1-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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