Thermo Fisher Scientific AKAP8/AKAP95 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP).
AKAP95, also known as AKAP8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP95 is recruited to the chromosomes and plays an essential role in mitotic progression. Characteristic of its family, AKAP95 participates in PKA signaling through an interaction with the RII regulatory subunit. In addition, AKAP95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.