Thermo Fisher Scientific AKAP8/AKAP95 Polyclonal Antibody
AKAP8/AKAP95 단백질을 표적하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot, IHC, IP 등 다양한 응용 가능. Rabbit IgG 기반으로 액상 형태이며 4°C에서 보관. 세포 내 PKA 신호 전달 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Item | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 642 and the C-terminus (residue 692) of human A-kinase anchor protein 8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase to specific sites within the cell. The R subunit (RII) of PKA interacts with RII-anchoring proteins, known as A-kinase anchoring proteins (AKAP).
AKAP95 (also known as AKAP8) is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP95 is recruited to chromosomes and plays an essential role in mitotic progression. It participates in PKA signaling through interaction with the RII regulatory subunit and forms a complex with HA95 and HDAC3, which is required for histone H3 deacetylation during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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