Thermo Fisher Scientific Bax Monoclonal Antibody (SP47)

Applications

Western Blot (WB)

• Tested Dilution: Not specified
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Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50
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Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is normal breast tissue.

Target Information

BAX is a member of the Bcl-2 family and plays an important role in the regulation of apoptosis.
Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX has a pro-apoptotic function.
Regulation of apoptosis involves both homo- and heterodimerization of different isoforms of BAX and Bcl-2.

The Bax gene encodes isoforms Bax alpha (21 kDa) and Bax beta (24 kDa). Both contain BH1, BH2, and BH3 domains; Bax beta has a unique carboxyl terminus lacking a hydrophobic transmembrane domain.
Bcl-2 also exists in multiple isoforms. Bcl-2 beta differs in the 3′ UTR and coding region compared to variant alpha, being shorter (22 kDa) and having a distinct C-terminus compared to Bcl-2 alpha (26 kDa).

Overexpression of BAX promotes cell death. BAX can form homodimers and heterodimerize with other BCL-2 related proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.