Thermo Fisher Scientific Bax Monoclonal Antibody (SP47)
Human Bax 단백질을 인식하는 Rabbit monoclonal antibody (Clone SP47). Western blot 및 IHC(P)에서 검증된 항체로, 단백질 A/G 정제 및 PBS 버퍼에 보관. 세포사멸(apoptosis) 연구용으로 적합하며, 열매개 항원 회수 권장.
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- MA516322
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- pk
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Applications
Western Blot (WB)
- Tested Dilution: Not specified
- View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50
- View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP47 |
| Immunogen | Synthetic peptide corresponding to internal region of human BAX protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537841 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is normal breast tissue.
Target Information
BAX is a member of the Bcl-2 family and plays an important role in the regulation of apoptosis.
Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX has a pro-apoptotic function.
Regulation of apoptosis involves both homo- and heterodimerization of different isoforms of BAX and Bcl-2.
The Bax gene encodes isoforms Bax alpha (21 kDa) and Bax beta (24 kDa). Both contain BH1, BH2, and BH3 domains; Bax beta has a unique carboxyl terminus lacking a hydrophobic transmembrane domain.
Bcl-2 also exists in multiple isoforms. Bcl-2 beta differs in the 3′ UTR and coding region compared to variant alpha, being shorter (22 kDa) and having a distinct C-terminus compared to Bcl-2 alpha (26 kDa).
Overexpression of BAX promotes cell death. BAX can form homodimers and heterodimerize with other BCL-2 related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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