
Thermo Fisher Scientific N-cadherin Monoclonal Antibody (SP90)
Rabbit monoclonal antibody (clone SP90) specific for human N-cadherin. Validated for IHC, IHC-P, and Flow Cytometry. Recommended dilution 1:100 (IHC-P), 1:20–1:100 (Flow). Supplied in PBS with 1% BSA and 0.1% sodium azide. Ideal for research on cell ad...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 | – |
| Flow Cytometry (Flow) | 1:20–1:100 | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP90 |
| Immunogen | Synthetic peptide corresponding to the C-terminus of human cadherin-N protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.005 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537843 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1 mM EDTA buffer (pH 8.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. After retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is mesothelioma tissue.
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion. It plays a crucial role in morphogenetic movements during development and regulates cell surface adhesion through homotypic interactions.
Its function depends on association with the actin cytoskeleton via interactions between its C-terminal region and cytoplasmic catenin proteins. This association is modulated by phosphorylation and dephosphorylation of beta-catenin.
N-cadherin contains five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It is essential during gastrulation and for establishing left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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