Thermo Fisher Scientific Adenovirus Type 2 Monoclonal Antibody (4D2)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Published species

Virus

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4D2

Immunogen

UV-irradiated Ad2 virus

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980963

Product Specific Information

MA5-11222 targets Adenovirus Fiber in IF and WB applications and shows reactivity with Virus samples.

The MA5-11222 immunogen is uV-irradiated Ad2 virus.

Target Information

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.