Thermo Fisher Scientific H3K4me3 Monoclonal Antibody (G.532.8)
H3K4me3 단일클론 항체(G.532.8)는 히스톤 H3의 N-말단 Lys4 삼중 메틸화 부위를 인식합니다. WB, IHC, ICC 등 다양한 응용에 적합하며, 비특이적 교차 반응이 없습니다. 고순도의 Affinity chromatography 정제 항체로 연구용에만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:4,000 | – |
| Immunocytochemistry (ICC/IF) | 1:400 | View 1 publication |
| Flow Cytometry (Flow) | – | View 1 publication |
| ChIP assay (ChIP) | – | View 2 publications |
| Peptide Array (Array) | 1:2,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Non-human primate, Rat, Yeast |
| Published Species | Bovine, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G.532.8 |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 121 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10977872 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several different types of post-translational modifications that influence cellular processes, including methylation, acetylation, and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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