Thermo Fisher Scientific H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified
H3K27me3 단백질의 trimethylation을 검출하는 rabbit monoclonal 항체. ChIP, WB, IHC 등 다양한 응용에 검증됨. 비특이적 교차반응 없음. 에피제네틱 연구 및 유전자 발현 조절 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:400 | - |
| Immunocytochemistry (ICC/IF) | 1:1,600 | View 2 publications |
| ChIP assay (ChIP) | 1–5 µg | View 3 publications |
| ChIP-sequencing (ChIP-Seq) | 3 µg | - |
| Peptide Array (Array) | 1:2,000 | - |
| CUT&RUN (C&R) | - | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G.299.10 |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 102 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11000749 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with mono-, di-, or tri-methylated histone H3 at Lys4, Lys9, Lys36, or histone H4 at Lys20.
- Click here for Master Lot linking information.
Target Information
H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. It is an epigenetic silencing mark associated with gene downregulation and heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and often opposes the activating mark H3K4me3. It is enriched in gene-rich regions linked to developmental regulators.
EZH2 (a PRC2 complex component) acts as the histone methyltransferase (writer) for H3K27me3, while UTX and JMJD3 serve as histone demethylases (erasers). Regulation of H3K27me3 is critical for gene expression control, and its dysregulation has been implicated in various diseases, including cancer.
H3K27me3 antibodies are widely used in Western blot and ChIP assays to study epigenetic modifications and chromatin occupancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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