Thermo Fisher Scientific H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

-

Immunocytochemistry (ICC/IF)

1:1,600

View 2 publications 2 publications

ChIP assay (ChIP)

1-5 µg

View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

3 µg

-

Peptide Array (Array)

1:2,000

-

CUT&RUN (C&R)

-

- -

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.299.10

Immunogen

Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

102 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11000749

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with mono-methylated, di-methylated or tri-methylated histone H3 at Lys4, Lys9, Lys36 or Histone H4 at Lys20.

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Target Information

H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.