Thermo Fisher Scientific TRIM28 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-852 detects KAP-1 (Kruppel-associated box-associated protein 1) in human, mouse, and rat samples.
It has been validated for use in Western blot, ICC/IF, and IHC (P) applications.
By Western blot, this antibody detects an approximately 87 kDa protein corresponding to recombinant human KAP-1, as well as a smaller band believed to represent a degradation product.
In immunofluorescent assays, this antibody primarily reacts with mouse or rat samples and may show non-specific staining in HeLa cells.
The PA1-852 antigen is recombinant human KAP-1.

Target Information

The KRAB (Kruppel-associated box) domain, approximately 45 amino acids long, is a transcriptional repression domain found in many transcription factors.
Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome.
These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835-amino-acid protein containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1).
KAP-1 targets KRAZ1 to centromeric heterochromatin regions containing HP1α, contributing to transcriptional repression.
Mutant KAP-1 proteins that bind KRAB but not HP1 lead to random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.