Thermo Fisher Scientific Neural/Glial Antigen 2 (NG2) Recombinant Rabbit Monoclonal Antibody (JM10-13)
인간 NG2 단백질을 인식하는 재조합 토끼 단클론 항체입니다. Western blot, IHC(P), Flow Cytometry 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 제조되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM10-13 |
| Immunogen | Synthetic peptide within Human NG2 aa 2271–2314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809856 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, followed by screening to select optimal clones for production.
Key advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The NG2 (CSPG4) protein is a human melanoma-associated chondroitin sulfate proteoglycan involved in stabilizing cell–substratum interactions during early melanoma cell spreading on endothelial basement membranes. CSPG4 is an integral membrane proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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