Thermo Fisher Scientific p53 Monoclonal Antibody (OTI6C1), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000
  Publications: [References not listed]

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation.
p53 activation leads to transcription of genes such as p21waf1, contributing to G1 or G2/M arrest. Structurally, p53 contains:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation correlates with tumor formation.
p53 plays a key role in preventing proliferation of damaged cells and functions as an anticancer factor.
Mutations in TP53 are common in human cancers and cause loss of tumor suppressor activity. Germline mutations are associated with Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.