Thermo Fisher Scientific T-bet/TBX21 Recombinant Rabbit Monoclonal Antibody (BLR110H)
T-bet/TBX21 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP에 사용 가능. 인간 반응성, 높은 특이성과 재현성 제공. 연구용으로 면역조절 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 0.5 µL/10⁶ cells |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR110H |
| Immunogen | Peptide representing a region between residue 485 and the C-terminus (residue 535) of Human T-box transcription factor TBX21 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development.
T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. It cooperates with Stat4 (signal transducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression.
Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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