Thermo Fisher Scientific XPF Monoclonal Antibody (OTI4E11), TrueMAB
ERCC4(XPF) 단백질을 표적으로 하는 Mouse 단일클론 항체로 Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 인간 및 생쥐 시료에 반응하며, carrier-free 형태로 제공되어 결합 실험에 활용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E11 |
| Immunogen | Full length human recombinant protein of human ERCC4 produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The structure-specific ERCC1/XPF endonuclease complex participates in the repair of two distinct types of DNA lesions:
- NER (Nucleotide Excision Repair) for UV-induced lesions and bulky chemical adducts
- Recombination repair for interstrand cross-links
In mammalian cells, XPG cleaves the 3′ side of the lesion, while ERCC1/XPF performs the 5′ incision. The ERCC1/XPF complex also likely processes partially repaired recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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