Thermo Fisher Scientific LC3B Polyclonal Antibody, HRP
LC3B 단백질을 인식하는 HRP 결합 폴리클로날 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간 및 여러 종에 반응하며, 자가포식 연구용으로 활용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria, Bovine, Dog, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 0.86 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Wet ice |
| RRID | AB_11154623 |
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and is important in neurogenesis.
Recent studies indicate that LC3B plays a vital role in autophagy, a process involving the bulk degradation of cytoplasmic components. Three human LC3 isoforms undergo post-translational modifications during autophagy.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells and is responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation.
LC3B mediates physical interactions between microtubules and components of the cytoskeleton, playing potential roles in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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