Thermo Fisher Scientific FOXP3 Polyclonal Antibody, Biotin
FOXP3 단백질을 타깃으로 하는 Biotin 결합 토끼 폴리클로날 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. 인간 및 생쥐 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 4℃ 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400–431). |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.74 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152305 |
Product Specific Information
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25+ CD4+ regulatory T cells, a low level in CD4+/CD25− cells, and is absent in CD4−/CD8+ T cells. FOXP3 may be a master regulatory gene and a specific marker of regulatory T cells.
Defects in the FOXP3 gene cause the scurfy phenotype in mice and are associated with IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome) in humans. Transcript variants encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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