Thermo Fisher Scientific p16INK4a Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide from near the amino terminus of human CDKN2A. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p16INK4a,uniProtId:P42771-1,ncbiNodeId:9606,antigenRange:1,antigenLength:156,antigenImageFileName:PA5-20379_p16INK4a_P42771-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20379_p16INK4a_P42771-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157205

Product Specific Information

A suggested positive control is mouse colon tissue lysate.

PA5-20379 can be used with blocking peptide PEP-0496.

Antibodies should not be exposed to prolonged high temperatures.

Target Information

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.