Thermo Fisher Scientific CD10 Recombinant Rabbit Monoclonal Antibody (RM337)
CD10 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체(RM337). WB, IHC, ICC/IF에 적합하며 높은 특이성과 민감도 제공. 로트 간 일관성, 동물 유래 성분 없음, 다양한 표적에 대한 광범위한 반응성 보유.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM337 |
| Immunogen | A peptide corresponding to the N-terminus of human CD10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744947 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA) is a cell surface enzyme with neutral metalloendopeptidase activity that inactivates various biologically active peptides.
It is expressed on:
- Lymphoblastic, Burkitt’s, and follicular germinal center lymphoma cells
- Immature B cells in adult bone marrow
- Cells from patients with chronic myelocytic leukemia (CML)
- Breast myoepithelial cells, bile canaliculi, fibroblasts
- Kidney and gut epithelial brush border cells
CD10 is a 100 kDa type II transmembrane glycoprotein encoded by a >45 kb gene. The 5’ untranslated region is alternatively spliced into four mRNA transcripts, with the coding region unaffected.
Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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