Thermo Fisher Scientific HSPA9 Monoclonal Antibody (30A5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:50

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus

Published species

Chicken, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

30A5

Immunogen

Grp75 isolated from human Molt4 cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSPA9,uniProtId:P38646-1,ncbiNodeId:9606,antigenRange:1-679,antigenLength:679,antigenImageFileName:MA1-91639_HSPA9_P38646-1_House_mouse.svg,antigenImageFileNamePDP:MA1-91639_HSPA9_P38646-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2120465

Product Specific Information

Western blot detects a band ~75 kDa. A suggested positive control is HeLa cell lysate (heat shocked).

Target Information

The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.