Thermo Fisher Scientific Active/Pro-Caspase 3 Monoclonal Antibody (31A1067)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

31A1067

Immunogen

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Active/Pro-Caspase 3,uniProtId:P42574-1,ncbiNodeId:9606,antigenRange:1-277,antigenLength:277,antigenImageFileName:MA1-91637_ActivePro-Caspase_3_P42574-1_House_mouse.svg,antigenImageFileNamePDP:MA1-91637_ActivePro-Caspase_3_P42574-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1954999

Product Specific Information

Predicted molecular weight: 31kDa.

Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer`s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.