Thermo Fisher Scientific Active/Pro-Caspase 3 Monoclonal Antibody (31A1067)
Active/Pro-Caspase 3 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단일클론 항체. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫드 등 반응성. 단백질 G 정제, 1 mg/mL 농도, PBS에 0.05% sodium azide 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 9 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:10–1:500 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC-F) | 1:10–1:500 | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | – |
| Flow Cytometry (Flow) | Assay-dependent | – |
| Immunomicroscopy (IM) | Assay-dependent | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 31A1067 |
| Immunogen | Full-length recombinant human caspase-3 protein. Recognizes an epitope in the large domain subunit of Caspase-3, detecting both pro Caspase-3 and the large subunit cleavage fragment. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1954999 |
Product Specific Information
- Predicted molecular weight: 31 kDa
- Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate
Target Information
This gene encodes a protein that belongs to the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing to produce two subunits (large and small) which dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9, and is itself processed by caspases 8, 9, and 10. It is the predominant caspase involved in cleavage of amyloid-beta 4A precursor protein, associated with neuronal death in Alzheimer’s disease. Alternative splicing results in two transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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