Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (16B1), Biotin, eBioscience
인간 CD144(VE-cadherin)을 인식하는 Biotin 결합 단클론 항체. 혈관 내피세포 연구에 적합하며 Flow Cytometry 및 ICC에 사용 가능. 0.5 mg/mL 농도의 액상 제품으로, 4°C에서 암소 보관. Affinity chromatography로 정제됨.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 5 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 12 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16B1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466611 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- PE
- PE-Cyanine7
- PE-eFluor 610
- Request custom conjugation
Product Specific Information
Description:
The 16B1 antibody reacts with human CD144 (VE-cadherin, cadherin-5). VE-cadherin is a calcium-dependent adhesion molecule localized at intercellular boundaries of endothelial cells in blood and lymphatic vessels. It plays a role in vascular permeability and remodeling.
Applications Reported:
Reported for use in flow cytometric analysis and immunocytochemistry.
Applications Tested:
Tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). Recommended ≤ 0.125 µg per test (100 µL final volume). Optimal dilution should be determined empirically.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
VE-cadherin is a calcium-dependent cell-cell adhesion glycoprotein in the cadherin superfamily, located on chromosome 16. It consists of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It mediates homophilic adhesion and is essential for endothelial cell junction organization. An alternative splice variant has been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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