Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), Biotin, eBioscience
마우스 CD150(SLAM)을 인식하는 9D1 클론 단일클론 항체로, Biotin 결합형입니다. Flow Cytometry에 적합하며, 활성화된 T 및 B 세포에서 발현되는 CD150 검출에 사용됩니다. 0.5 µg/test 권장, 4°C 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9D1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466613 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugates:
Product Specific Information
The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T (especially TH1) and B cells, and its expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.
Signaling through SLAM in T cells induces proliferation and enhances interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells. The 9D1 antibody is reported to be activating.
Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, antibody clone mShad150 is recommended (Product # 12-1502).
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymic and splenic cells; optimal at ≤0.5 µg/test (100 µL reaction volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Target Information
CD150 (SLAM) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. It is constitutively expressed on peripheral blood memory T cells, immature thymocytes, and subsets of B cells, and is rapidly induced after activation.
Activated B cells express both membrane-bound and soluble forms of SLAM. SLAM-SLAM interactions enhance B cell expansion and differentiation. The extracellular domain of CD150 also serves as a receptor for the measles virus and co-activator on T and B cells.
CD150 interacts with SH2D1A and PTPN11 via its cytoplasmic domain, and mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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