Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 1 publication

Product Specifications

Available Conjugates:

• APC
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T (especially TH1) and B cells, and its expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.

Signaling through SLAM in T cells induces proliferation and enhances interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells. The 9D1 antibody is reported to be activating.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, antibody clone mShad150 is recommended (Product # 12-1502).

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymic and splenic cells; optimal at ≤0.5 µg/test (100 µL reaction volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Target Information

CD150 (SLAM) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. It is constitutively expressed on peripheral blood memory T cells, immature thymocytes, and subsets of B cells, and is rapidly induced after activation.

Activated B cells express both membrane-bound and soluble forms of SLAM. SLAM-SLAM interactions enhance B cell expansion and differentiation. The extracellular domain of CD150 also serves as a receptor for the measles virus and co-activator on T and B cells.

CD150 interacts with SH2D1A and PTPN11 via its cytoplasmic domain, and mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.