Thermo Fisher Scientific NFYB Polyclonal Antibody
Rabbit polyclonal antibody targeting NFYB for WB and IHC applications. Reacts with human, mouse, and rat. Affinity purified with >95% purity. Supplied as liquid in PBS with 50% glycerol. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminal of Human NFYB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2718955 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y consists of three subunits: NF-YA, NF-YB, and NF-YC, all of which are necessary for DNA binding.
Each NF-Y subunit contains a conserved segment required for NF-Y–DNA complex formation, homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of NF-YB and NF-YC interact through a histone-fold “handshake” motif, analogous to histone proteins H2B and H2A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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