Thermo Fisher Scientific SETD2 Monoclonal Antibody (OTI1E1), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

SETD2 is a histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate.
It serves as the primary enzyme generating H3K36me3, a marker for epigenetic transcriptional activation.
SETD2 plays a crucial role in:

• Chromatin structure modulation during transcription elongation
• DNA mismatch repair by recruiting the MutS alpha complex
• Maintenance of heterochromatin via DNMT3A recruitment
• Coupling of splicing and RNA polymerase elongation
• Angiogenesis regulation
• Regulation of adenovirus 12 E1A gene promoter activity upon infection

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.