Thermo Fisher Scientific p53 Monoclonal Antibody (OTI5E2), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. p53 upregulation leads to activation of genes such as p21waf1, contributing to G1 or G2/M arrest.

Structurally, p53 contains:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation has been associated with human tumor formation. p53 plays a key role in preventing proliferation of damaged cells and acts as an anticancer agent. It is expressed at low levels in normal cells but highly expressed in transformed cells, often contributing to malignancy. Mutations in the TP53 gene can result in loss of tumor suppressor function and are linked to various human cancers, including Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.