Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, NovaFluor Blue 510, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

NovaFluor™ Blue 510 NovaFluor™ Blue 510 NovaFluor™ Blue 510

View additional formats

• [AP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Blue 555](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 555/host/Goat/)
• [NovaFluor Blue 585](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 585/host/Goat/)
• [NovaFluor Blue 610-70S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 610-70S/host/Goat/)
• [NovaFluor Blue 660-120S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 660-120S/host/Goat/)
• [NovaFluor Yellow 590](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 590/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/PE/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

493/513 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_3099277 (AB_2927364)

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: This is affinity purified HRP-conjugated Goat Anti-Rat IgG (H+L). This antibody has minimal cross-reactivity to Mouse, Human, Bovine, Horse, and Rabbit Serum Proteins. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rat IgG and with thelight chains common to most rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: HRP anti-rat IgG has been reported for use in immunoblotting (WB), immunohistochemical staining, and ELISA.

Applications Tested: This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, human,bovine, horse, and rabbit serum proteins, but the antibody may cross-react with immunoglobulins from other species.Suggested dilution ranges are:1:500 - 1:5,000 for immunohistochemistry on tissue sections 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue) Laser

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.