Thermo Fisher Scientific Phospho-Chk2 (Thr68) Recombinant Rabbit Monoclonal Antibody (Chk2T68-D12)
인산화된 Chk2(Thr68)를 검출하는 재조합 토끼 단클론 항체로 높은 특이성과 민감도를 제공. Western blot 및 유세포분석에 적합하며, 로트 간 일관성 보장. HEK293 세포 발현, PBS 기반 저장용액, -20°C 보관.
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Applications
Western Blot (WB)
- Assay-dependent
Flow Cytometry (Flow)
- 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Chk2T68-D12 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human phospho Chk2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745002 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are linked to cancer. CHK2 is activated by DNA damage and phosphorylates modulators of cell cycle control, including tumor suppressor proteins. Chk2 can phosphorylate BRCA1, enabling BRCA1 to restore survival after DNA damage.
Chk2 acts as a tumor suppressor protein, with gene mutations associated with Li-Fraumeni syndrome and predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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