Thermo Fisher Scientific Midkine Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine. Anti-Human Midkine-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hMidkine by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, used with PeproTech Polyclonal Anti-Human Midkine (500-P171) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hMidkine.

Western Blot:
To detect hMidkine by Western Blot analysis, use at 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant hMidkine is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P171BT-1MG will be provided as 2 × 500 µg.

Target Information

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting its role in tumorigenesis, potentially through angiogenic effects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.