Thermo Fisher Scientific Nucleophosmin Monoclonal Antibody (NPM1, 3286)
Nucleophosmin 단백질 검출용 Mouse monoclonal antibody로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. Recombinant human NPM1 (aa185-287) 면역원 사용. 고순도 Protein A/G 정제, PBS buffer에 보존, 연구용으로 적합.
- 카탈로그번호
- 4869-MSM6-P1ABX
- 판매단위
- pk
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Thermo Fisher Scientific Nucleophosmin Monoclonal Antibody (NPM1, 3286)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NPM1, 3286 |
| Immunogen | Recombinant human NPM1 protein fragment (aa185–287) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody tested in direct ELISA (use at 2–4 µg/mL for coating).
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- For formalin-fixed tissues: heat sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at room temperature for 20 min.
Target Information
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly and transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and p53 regulation.
It shuttles between the nucleus, cytoplasm, and nucleolus, chaperoning core histones. NPM1 binds nucleic acids, prevents protein aggregation, protects enzymes during thermal denaturation, and aids in renaturation of chemically denatured proteins.
NPM1 interacts with tumor-associated chromosome translocations forming fusion proteins such as NPM-ALK, NPM-RAR, and NPM-MLF1, acting as an interface for oligomerization and oncogenic conversion.
Dysfunction of NPM1 is associated with diseases including acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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