Thermo Fisher Scientific Phospho-MEK3 (Ser189) Polyclonal Antibody
Phospho-MEK3 (Ser189) Polyclonal Antibody는 인간, 마우스, 랫트에서 인산화된 MEK3(Ser189)를 특이적으로 검출합니다. Western blot, IHC, ICC/IF에 적합하며 고순도 정제 및 안정한 PBS/glycerol 저장 버퍼를 사용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP2K3 (Accession P46734), corresponding to amino acid residues around phosphorylated Ser189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816385 |
Product Specific Information
Antibody detects endogenous levels of MKK3 only when phosphorylated at Serine 189.
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways and is necessary for glucose transporter expression. It phosphorylates and activates MAPK14/p38-MAPK, and can be activated by insulin. Expression of the RAS oncogene leads to accumulation of the active form of this kinase, resulting in constitutive activation of MAPK14 and oncogenic transformation of primary cells. Inhibition of this kinase is implicated in the pathogenesis of Yersinia pseudotuberculosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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