Thermo Fisher Scientific DNMT1 Monoclonal Antibody (4G11-C7), APC
DNMT1 단백질을 검출하기 위한 APC-conjugated mouse monoclonal antibody입니다. Western blot, IHC, IP, ChIP 등 다양한 응용에 사용 가능하며, Human, Mouse, Zebrafish 등에 반응합니다. 단백질 G로 정제되었으며 4°C에서 보관합니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution | 참고 |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | 1:1,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fish, Human, Mouse, Zebrafish |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G11-C7 |
| Immunogen | Raised against a partial protein corresponding to amino acids 620–950 of human DNMT1 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931748 |
Additional Formats
- FITC (Cat. No. MA5-45295)
- PerCP (Cat. No. MA5-45296)
- Custom conjugation available upon request
Product Specific Information
- 2 µg/mL of MA5-45294 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 180 kDa.
- Cross-reacts with mouse DNMT1.
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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