Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PE, eBioscience
마우스 IL-13을 인식하는 eBio13A 단클론 항체로, PE 형광 표지된 제품입니다. Flow cytometry 및 ELISA, ELISPOT에 적합하며, 알레르기 염증 및 면역 조절 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 78 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio13A |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852712 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 660
- Functional Grade
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The eBio13A antibody reacts with mouse IL-13, a cytokine produced mainly by Th2 cells and antigen-primed CD8 T cells. IL-13 plays a key role in allergic inflammation, parasitic clearance, and immune regulation, showing both inhibitory and stimulatory activity in cancer models.
Applications Reported: Useful for ELISA, ELISPOT capture, and intracellular staining for flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining of cultured mouse splenocytes. Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation/Emission: 488–561 nm / 578 nm (Blue, Green, Yellow-Green lasers)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-13 is an immunoregulatory cytokine produced by activated Th2 cells that modulates inflammatory and immune responses. It down-regulates macrophage activity, inhibits pro-inflammatory cytokine production, and plays a role in allergen-induced asthma independent of IgE and eosinophils. IL-13 also regulates B cell maturation and promotes IgE isotype switching.
Structurally similar to IL-3, IL-4, IL-5, and GM-CSF, the IL-13 gene is located on chromosome 5q within a cytokine gene cluster near IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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