Thermo Fisher Scientific Avidin Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 1:2000–1:5000

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 1 publication

Product Specifications

Product Specific Information

PA1-26844 detects Avidin and does not react with Streptavidin.
Store product as a concentrated solution and centrifuge briefly prior to opening the vial.

Target Information

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag frequently used for immunodetection methods.
This protein contains four identical subunits with a combined molecular mass of 67,000–68,000 Da. Each subunit binds one molecule of biotin, involving tryptophan and lysine in the binding site.
The subunit consists of 128 amino acids and has an isoelectric point of 10–10.5. Avidin is highly soluble in water and salt solutions, stable over a wide range of pH and temperatures, and resistant to chemical modification.
These properties make avidin useful for detection and protein purification. Pure avidin is a key component in certain IHC methods. Alkaline phosphatase (AP) and HRP conjugates of avidin are useful for Western blotting or ELISA detection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.