Thermo Fisher Scientific Avidin Polyclonal Antibody, HRP
Avidin에 특이적인 HRP 결합 폴리클로날 항체로 Western blot 및 IHC에 사용 가능. 닭 유래 Avidin을 면역원으로 제작된 Rabbit IgG 형 항체. 고농도(10 mg/mL) 액상 형태로 제공되며 4°C 또는 -20°C 보관. Streptavidin에는 반응하지 않음.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Avidin Polyclonal Antibody, HRP
Applications
Western Blot (WB)
- Tested Dilution: 1:2000–1:5000
Immunohistochemistry (IHC)
- Tested Dilution: Not specified
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Avidin from hen egg white |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 10 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_779340 |
Product Specific Information
PA1-26844 detects Avidin and does not react with Streptavidin.
Store product as a concentrated solution and centrifuge briefly prior to opening the vial.
Target Information
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag frequently used for immunodetection methods.
This protein contains four identical subunits with a combined molecular mass of 67,000–68,000 Da. Each subunit binds one molecule of biotin, involving tryptophan and lysine in the binding site.
The subunit consists of 128 amino acids and has an isoelectric point of 10–10.5. Avidin is highly soluble in water and salt solutions, stable over a wide range of pH and temperatures, and resistant to chemical modification.
These properties make avidin useful for detection and protein purification. Pure avidin is a key component in certain IHC methods. Alkaline phosphatase (AP) and HRP conjugates of avidin are useful for Western blotting or ELISA detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.