Thermo Fisher Scientific Avidin Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

PA1-26812 detects Avidin. PA1-26812 will not react with streptavidin.

Target Information

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag frequently used to label antibodies and other probes for immunodetection methods. This protein contains four identical subunits with a combined molecular mass of 67,000–68,000 daltons. Each subunit binds one molecule of biotin, and studies show that tryptophan and lysine are involved in the biotin binding site. The subunit consists of 128 amino acids, with an isoelectric point of 10–10.5. Avidin is highly soluble in water and salt solutions and remains stable over a wide range of pH and temperatures. Extensive chemical modification has little effect on its activity, making it useful for detection and protein purification. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.