Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (MR-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

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Immunohistochemistry (IHC)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

4 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG1

Class

Monoclonal

Type

Antibody

Clone

MR-1

Immunogen

Activated mouse Th1 clone D1.6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153361

Product Specific Information

This antibody detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Functional Application: Blocking.

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.