Thermo Fisher Scientific Phospho-c-Raf (Ser43) Recombinant Rabbit Monoclonal Antibody (SD087-05)
Phospho-c-Raf (Ser43) 인산화 단백질을 검출하기 위한 재조합 토끼 단클론 항체. Western blot에 최적화되어 있으며, 사람, 마우스, 랫에서 반응. 높은 특이성과 일관된 로트 간 재현성 제공. 단백질 안정성을 위해 -20°C 보관 권장.
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Applications
- Western Blot (WB): 1:1,000–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD087-05 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser43 of Human Raf1 (aa 20–69) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809672 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
These antibodies provide:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of Ras family GTPases. Once activated, Raf-1 phosphorylates and activates MEK1/2, which in turn activate ERK1/2.
Activated ERKs regulate gene expression related to cell division, apoptosis, differentiation, and migration.
Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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