Thermo Fisher Scientific CD8 Polyclonal Antibody, Alexa Fluor 647
Alexa Fluor 647로 표지된 CD8 폴리클로날 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. Rabbit IgG 기반으로 Protein A 정제, 고농도(1 mg/mL) 액상 형태로 제공됩니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from mouse CD8 (amino acids 51–150) |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family, plays an integral role in signal transduction, T cell differentiation, and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
Its counterpart, CD4, acts as a co-receptor for MHC class II molecules. CD8α is essential for binding to MHC-I and is also expressed as a homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells.
Engagement of MHC-I/peptide complexes on antigen-presenting cells triggers recruitment of Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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