Thermo Fisher Scientific CD34 Polyclonal Antibody
CD34 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. 인간, 생쥐, 토끼 등 여러 종에 반응하며, Protein A 정제 및 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Goat, Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human CD34, amino acids 301–385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It functions as a stem cell marker and may act as a receptor involved in endocytosis, adhesion, differentiation, and proliferation of hematopoietic stem and progenitor cells. CD34 expression represents a specific developmental state with altered adhesion properties. It may mediate stem cell attachment to the bone marrow extracellular matrix or stromal cells and serve as a scaffold for glycan attachment, facilitating interactions with lectins. The intracellular domain can be phosphorylated by protein kinase C, suggesting a role in signal transduction. CD34 dysfunction has been associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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