Thermo Fisher Scientific Perforin Monoclonal Antibody (dG9)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive control: Spleen
• Cellular location: Secreted, Cell membrane

Target Information

Perforin is a major cytolytic protein found in cytolytic granules. It acts as a cytolytic mediator released by cytotoxic lymphocytes to defend against tumors and viral infections. The protein consists of 555 amino acids, including a 21 amino acid signal peptide, with a molecular weight of approximately 70–75 kDa. Perforin functions as a pore-forming protein, similar in mechanism to complement component C9, with demonstrated homology between the two.

Perforin expression is restricted to killer cell lines and absent in helper T lymphocytes or other tumor cells. It is a key effector molecule in T-cell and natural killer (NK) cell-mediated cytolysis. Mutations in the perforin gene are associated with familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.