Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (ZY184)
인간 ZAP-70 단백질을 인식하는 단클론 항체로, T세포 및 NK세포의 신호전달 연구에 적합합니다. 파라핀 포매 조직 면역조직화학(IHC-P)에 사용 가능하며, 단백질 A로 정제된 액상 형태입니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Immunohistochemistry (Paraffin) (IHC-P)
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZY184 |
| Immunogen | Recombinant protein of human ZAP-70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human lymph node
- Cellular location: Cytoplasm, membrane
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer (NK) cells and is essential for T-cell receptor (TCR) activation. Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319 within the interdomain B region between the SH2 and kinase domains. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP-70 function in lymphocyte signaling, while phosphorylation at tyrosine 292 terminates activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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