Thermo Fisher Scientific TRPC6 Monoclonal Antibody (3F2.H10.F2)
인간 TRPC6 단백질을 인식하는 Mouse 모노클로날 항체로, WB, IHC, ELISA, IP 등에 사용 가능. 단백질 A로 정제된 액상 형태이며, -20°C에서 보관. 인간 및 침팬지 TRPC6에 반응하며 연구용으로 적합.
- 카탈로그번호
- 200-301-B59x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TRPC6 Monoclonal Antibody (3F2.H10.F2)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2.5 µg/mL |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chimpanzee, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F2.H10.F2 |
| Immunogen | Synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for human TRPC6 protein. A BLAST analysis indicates cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
Target Information
Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation occurs independently of protein kinase C and is not triggered by low intracellular calcium levels.
Defects in the Trpc6 gene are associated with focal segmental glomerulosclerosis 2 (FSGS2). Trpc6 belongs to the mammalian transient receptor potential (TRP) superfamily, specifically the canonical TRP (TRPC) family. Members of this family are components of receptor-regulated cation channels in various cell types. The TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting activation via PLC-coupled receptors.
When expressed in transfected cells, Trpc6 acts as a non-selective, store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and insensitive to IP3 activation. Evidence suggests that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo. Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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