Thermo Fisher Scientific HERC5 Recombinant Rabbit Monoclonal Antibody (8H23L10)
HERC5 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 면역침전(IP)에 사용 가능. 높은 특이성과 민감도를 제공하며, 로트 간 일관성이 우수함. 단기간 4°C, 장기간 -20°C 보관 권장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H23L10 |
| Immunogen | Protein corresponding to human HERC5 [aa1–aa141] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2784598 |
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Sheep.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
HERC5 (E3 ISG15 - protein ligase HERC5) is a major E3 ligase for ISG15 conjugation and acts as a positive regulator of the innate antiviral response induced by interferon.
It functions as part of the ISGylation machinery that recognizes target proteins broadly and catalyzes ISGylation of IRF3, leading to sustained activation.
HERC5 also attenuates IRF3-PIN1 interaction, which prevents IRF3 ubiquitination and degradation, thereby enhancing antiviral response.
It is physically associated with polyribosomes and modifies newly synthesized proteins cotranslationally.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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