Thermo Fisher Scientific DFF35/DFFA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Monkey

Target Information

ICAD is the inhibitor of caspase-3-activated DNase, a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45 kDa (ICAD-L/DFF45) and a 35 kDa isoform (ICAD-S/DFF35). Both isoforms can bind and inhibit CAD, but only ICAD-L is functional. ICAD is cleaved and inactivated to allow caspase-activated DNase (CAD) to execute nuclear apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with ICAD. Activation occurs through caspase-3-mediated cleavage at residues 117 and 224, releasing ICAD fragments from CAD. ICAD also acts as a CAD-specific folding chaperone. Recent studies suggest ICAD as a potential target for restoring apoptotic signal transduction in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been identified for the ICAD gene.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.