Thermo Fisher Scientific Phospho-DOK1 (Tyr398) Polyclonal Antibody
Phospho-DOK1 (Tyr398) 특이적 폴리클로날 항체로, 인간·마우스·랫트 시료에서 인산화된 DOK1 단백질 검출에 사용됩니다. Western blot 및 Immunocytochemistry에 적합하며, 고순도 정제 및 안정한 PBS/glycerol buffer로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DOK1 (Accession Q99704), corresponding to amino acid residues around phosphorylated Tyr398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816502 |
Product Specific Information
This antibody detects endogenous levels of p62 Dok only when phosphorylated at Tyrosine 398.
Target Information
DOK1 (P62dok, Docking protein 1) is a cytoplasmic adaptor protein that down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and migration.
It is a major substrate for tyrosine kinases such as c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation, DOK1 forms a complex with ras GTPase-activating protein.
Phosphorylation by the insulin receptor kinase negatively regulates the insulin signaling pathway. DOK1 contains a pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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