Thermo Fisher Scientific TNNC1 Monoclonal Antibody (UMAB204), UltraMAB
Human TNNC1 단백질을 인식하는 Mouse monoclonal antibody (Clone UMAB204). Western blot, IHC, ChIP, Peptide Array 등에 사용 가능. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| ChIP assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB204 |
| Immunogen | Full length human recombinant protein of human TNNC1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Troponin (Tn) is the central regulatory protein of striated muscle contraction. It consists of three components:
- Tn-I: Inhibitor of actomyosin ATPase
- Tn-T: Contains the binding site for tropomyosin
- Tn-C: Binds calcium ions
The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. This calcium-ion uptake by troponin-C induces a series of allosteric changes in the muscle thin filament, enabling actin–myosin interaction, ATP hydrolysis, and tension generation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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