Thermo Fisher Scientific Human IL-10 Recombinant Protein, eBioscience

Thermo Fisher Scientific Human IL-10 Recombinant Protein (eBioscience)

Applications

• Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Description:
Interleukin-10 (IL-10), also known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.6 kDa protein composed of 160 amino acids. It shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI.
IL-10 exhibits multiple biological activities, including:

• Inhibition of macrophage-mediated cytokine synthesis
• Suppression of delayed-type hypersensitivity response
• Stimulation of Th2 cell response leading to increased antibody production

Source:
Produced in E. coli.

Purity:
Greater than 95% by SDS-PAGE and HPLC analyses.

Reconstitution:
Quick spin followed by reconstitution in sterile distilled water to a concentration not less than 100 µg/mL.
This solution can be diluted into aqueous buffers and stored at 4°C for 1 week or -20°C for long-term storage.
For in vitro applications, IL-10 is biologically active at concentrations of 0.2–20.0 ng/mL.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes and, to a lesser extent, lymphocytes. It plays a key role in immunoregulation and inflammation by:

• Down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages
• Enhancing B cell survival, proliferation, and antibody production
• Blocking NF-kappa B activity
• Regulating the JAK-STAT signaling pathway

Knockout studies in mice indicate IL-10 is an essential immunoregulator in the intestinal tract.
Mutations in IL10 are associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Diseases linked to IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Note:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.