Thermo Fisher Scientific Hepatitis B virus core Ag Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

ELISA (ELISA)

0.01-0.1 µg/mL

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from internal sequence (31-178aa) of human hepatitis B Core Antigen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Hepatitis B Core Antigen

Cellular location: Nuclei of infected cells.

Target Information

The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. Hepatitis B virus is spherical in shape with a diameter of 42nm. It contains a 27 nm partially double stranded DNA core enclosed within a lipoprotein coat. The antigenic activity of the nucleocapsid core is designated as hepatitis B core antigen. The antigens in the outer surface are called as hepatitis B virus surface antigens. Core antigens are localized within the nuclei whereas the surface antigens are present in the cytoplasm of the infected cells. Antibodies to surface antigens appear in circulation at an early stage of infection whereas the antibodies to the core antigens are detected after several weeks. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries. Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.