Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr493, Tyr526) Recombinant Rabbit Monoclonal Antibody (Zap70Y493-H11)
ZAP-70/Syk 인산화 형태(Tyr493, Tyr526)를 검출하는 Thermo Fisher recombinant rabbit monoclonal antibody. Western blot 및 유세포 분석에 적합하며, 높은 특이성과 민감도, 로트 간 일관성을 제공. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Assay-dependent
Flow Cytometry (Flow)
- 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Zap70Y493-H11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2896850 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase and a member of the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation.
Upon T-cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B, located between the SH2 and kinase domains.
Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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