Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr493, Tyr526) Recombinant Rabbit Monoclonal Antibody (Zap70Y493-H11), PE

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL / 1×10⁶ cells

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and the best clones are selected for production.
Advantages include:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the rabbit immune repertoire

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed in T-cells and natural killer cells and is essential for T-cell receptor activation.
Upon TCR engagement, ZAP70 is phosphorylated on Tyr292, Tyr315, and Tyr319 in the interdomain B region between the SH2 and kinase domains.

• Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhance ZAP70-mediated lymphocyte signaling.
• Tyr292 phosphorylation attenuates signaling by terminating transient activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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