Thermo Fisher Scientific Erythropoietin (EPO) Monoclonal Antibody (EPO, 1368)
인간 EPO 단백질을 인식하는 mouse IgG1 monoclonal antibody로, Western blot, IHC, Peptide Array에 적합합니다. 고순도 Protein A/G 정제, 액상 형태로 제공되며 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EPO, 1368 |
| Immunogen | Recombinant fragment (around aa 28–162) of human EPO protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Erythropoietin (EPO) is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. It induces the final maturation of partially differentiated erythroid cells and promotes production of red cell-specific proteins such as hemoglobin.
The EPO receptor (EPOR) is highly expressed on precursor cells, with expression decreasing as cells mature. During fetal development, hepatocytes are the main source of EPO, whereas in adults it is produced by renal interstitial cells.
EPO expression increases under hypoxic conditions and may also play a role in neuronal tissue protection and repair initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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